For example, the purification of cortisone and its observed anti-inflammatory effects, the isolation of thyroxine and its upregulation of pulse and metabolism, as well as the extraction of pancreatic insulin and its initiation of anti-diabetic activity. These hormones were known to be associated with a variety of diseases, and by the s glucocorticoids were commonly used therapeutically.
However, the targets of these hormones remained intangible, and it was not until a few key experiments that hormones could be linked to transcriptional control. Radiolabeled-hormones enabled the discovery of cytoplasmic proteins that would translocate to the nucleus upon binding estradiol in uterine tissue. Similar work in Drosophila showed that pulses of ecdysone, the insect molting hormone, trigger subsequent chromosomal puffing activity.
A host of studies in the s and early s identified high affinity receptors for the steroid hormones as well as their ability to associate with and manipulate the genome. Soon after, the discoveries of the retinoic acid receptor and the insect ecdysone receptor made the concept of an evolutionarily conserved nuclear receptor superfamily concrete.
With almost 50 nuclear receptor genes in the human genome, this superfamily displays a vast diversity in ligand, function and transcriptional mechanism. Molecular Biology.
Transcription Factors. Research Personnel.
The Nuclear Receptor Superfamily
Gene Expression. Methods in Molecular Biology , In: Methods in Molecular Biology , Vol. In The Nuclear Receptor Superfamily: Methods and Protocols, expert researchers describe a range of molecular, structural and cell biological techniques currently used to investigate the structure-function of nuclear receptors, together with experimental approaches that may lead to new therapeutic strategies for treating nuclear receptor-associated diseases.
Written in the highly successful Methods in Molecular Biology TM series format, the chapters in this volume contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as notes from the experts to highlight tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, The Nuclear Receptor Superfamily: Methods and Protocols provides beneficial and time-saving guidance for all those undertaking research in this ever-growing field of study.
- Nuclear Receptor Analysis Luciferase Vectors;
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